lytD [2017-8-11 16:0:3]

peptidoglycan N-acetylglucosaminidase

locus: BSU35780

pI: 9.94

mw: 95.36 kDa

protein length: 880 aa

gene length: 2640 bp

function: major autolysin, cell separation

product: peptidoglycan N-acetylglucosaminidase

essential: no

ec: 3.2.1.96

synonyms: cwlG

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.3|Cell wall degradation/ turnover] → [category|SW 1.1.3.1|Autolysis]
• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.3|Cell wall degradation/ turnover] → [category|SW 1.1.3.5|N-acetyl-β-D-glucosaminidases]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

Gene

Coordinates: 3,684,826 → 3,687,468

The protein

Catalyzed reaction/ biological activity

• hydrolysis of the glycosidic bond between N-acetyl-β-d-glucosamine residues and adjacent monosaccharides [Pubmed|18266855]

Protein family

• glycosyl hydrolase 73 family (according to Swiss-Prot)

[SW|Localization]

• extracellular (signal peptide) [Pubmed|18957862]

Expression and Regulation

Operons

• genes: [gene|29219315D31BA4ADE41687EFF17FE41D6C23D157|lytD]
  description: [Pubmed|7934877]

  sigma factors

  • [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD]: sigma factor, [Pubmed|7934877], in [regulon|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD regulon]

Biological materials

Mutant

• 1A788 ( ''lytD''::''tet''), [Pubmed|7934877], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A788&Search=1A788 BGSC]
• 1A792 ( ''lytD''::''tet''), [Pubmed|1588906], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A792&Search=1A792 BGSC]

References

Reviews

• 18266855

Original publications

• 7934877,19542270,18957862,21821766